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| ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安. |
| The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23... |
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Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023 |
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| Santos, Paulo Augusto Almeida; Paiva, Renato; Silva, Luciano Coutinho; Souza, Ana Cristina de; Santana, Marlucia Cruz de; Silva, Diogo Pedrosa Corrêa da. |
| The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.00.00.00-4 - ciências agrárias; 2.03.03.00-9 - fisiologia vegetal; Native species; In vitro conservation; Recalcitrant seeds; Plant genetic diversity conservation; Vitrification; Droplet vitrification Conservação in vitro; Criopreservação vegetal. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573 |
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| Hajarian,H; Wahid,H; Rosnina,Y; Daliri,M; Dashtizad,M; Karamishabankareh,H; Abas Mazni,O. |
| The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Immature oocyte; Vitrification; Cryodevice. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100011 |
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| MOZDARANI,HOSSEIN; MORADI,SHABNAM Z. |
| This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration... |
| Tipo: Journal article |
Palavras-chave: Cryopreservation; Vitrification; Mouse embryo; Viability; Chromosome abnormalities. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004 |
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| Pradieé,J.; Cardoso,T.F.; Silva,E.F.; Gonçalves,A.O.; Gastal,G.D.A.; Rosa,C.E.; Mondadori,R.G.; Pegoraro,L.M.C.; Vieira,A.D.; Lucia Jr.,T.. |
| ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sheep embryos; Antioxidant; Reactive oxygen species; Frozen ram sperm; Vitrification. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309 |
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| Villamil,Paula Rodriguez; Ongaratto,Felipe Ledur; Silva,Daniela Scherer da; Rodrigues,Berenice de Avila; Rodrigues,Jose Luiz. |
| The aim of this study was to determine the effect of dimethylformamide (DF) associated with ethylene glycol (EG) or 1-2 propanediol (PROH) during vitrification, on the in vitro development of mouse blastocysts. Cryoprotectant toxicity was evaluated exposing embryos into three different equilibrium solutions (ES) composed by DF, EG or PROH mixtures (10% v/v of each) in mPBS + 0.5% PVA at different interval times (1, 3 and 10min). In a second experiment, embryos were exposed to the same ES (either 1 or 3min), following for the three respectively vitrification solutions (VS) (20% v/v of each) for 30s. After 72 hours of in vitro culture, embryo hatching and expansion rates were similar for the ES1 and ES2 equilibration solutions during the time interval of 1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Blastocyst; Vitrification; Dimethylformamide; Glass micro pipettes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100022 |
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| Santos,Rodrigo Marques dos; Barreta,Marcos Henrique; Frajblat,Marcel; Cucco,Diego Córdova; Mezzalira,Joana Claudia; Bunn,Silvério; Cruz,Fabiano Buss; Vieira,Arnaldo Diniz; Mezzalira,Alceu. |
| The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitrification; Cryopreservation; Oocytes; Bovine; Vacuum. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024 |
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